Stabilization of plakoglobin and enhanced keratinocyte cell-cell adhesion by intracellular O-glycosylation.
J Biol Chem
; 281(18): 12786-91, 2006 May 05.
Article
in En
| MEDLINE
| ID: mdl-16510446
ABSTRACT
O-Glycosylation modifies and regulates a variety of intracellular proteins. Plakoglobin, which functions in both cell-cell adhesion and signal transduction, is modified by O-glycosylation; however, the significance is unknown. To investigate the functional consequence of plakoglobin O-glycosylation, we cloned and overexpressed in keratinocytes murine O-GlcNAc transferase (mOGT). Over expression of mOGT in murine keratinocytes resulted in (i) glycosylation of plakoglobin and (ii) increased levels of plakoglobin due to post-translational stabilization of plakoglobin. Additionally, overexpression of mOGT in keratinocytes correlated with increased staining for cell-cell adhesion proteins and greater cell-cell adhesion. These observations suggest that O-glycosylation functions to regulate the post-translational stability of plakoglobin and keratinocyte cell-cell adhesion.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Keratinocytes
/
Gamma Catenin
Limits:
Animals
Language:
En
Journal:
J Biol Chem
Year:
2006
Document type:
Article
Affiliation country:
United States