Expression, purification and crystallization of Streptococcus dysgalactiae-derived mitogen.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 62(Pt 3): 242-4, 2006 Mar 01.
Article
in En
| MEDLINE
| ID: mdl-16511312
ABSTRACT
Superantigens are bacterial or viral toxins with potent immunostimulatory properties. Streptococcus dysgalactiae-derived mitogen, a 25 kDa protein, is a recently discovered superantigen isolated from S. dysgalactiae culture supernatant. Sequence considerations suggest that it belongs to a new superantigen family distinct from other superantigens. The protein was expressed in Escherichia coli cells and purified to homogeneity. Crystals were grown at pH 4.2-4.4 in the presence of 18-20%(w/v) PEG 3350 and 0.4 M lithium nitrate. A complete data set to 2.4 A resolution was collected from a single crystal at liquid-nitrogen temperatures using synchrotron radiation. The crystals belong to space group P3/P3(1)/P3(2), with unit-cell parameters a = b = 52.7, c = 62.4 A, gamma = 120 degrees and one molecule in the crystallographic asymmetric unit.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Streptococcus
/
Superantigens
/
Mitogens
Language:
En
Journal:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Year:
2006
Document type:
Article
Affiliation country:
Finland