Slower cycling of nestin-positive cells in neurosphere culture.
Neuroreport
; 17(4): 377-81, 2006 Mar 20.
Article
in En
| MEDLINE
| ID: mdl-16514362
ABSTRACT
Neural stem cells are multipotent and self-renewing cells with important potential application in cell replacement therapy in brain damage. Many studies have shown that nestin-positive cells represent neural stem and progenitor cells in the central neural system. Here, we derived neural stem cells from the subventricular zone of a newborn nestin-promoter-driven green fluorescent protein mouse, and found that the percentage of nestin-positive cells decreased continuously at each passage in neurosphere culture. Using the relative proliferation ratio and relative division ratio analysis, we concluded that the slower cycling of nestin-positive cells was responsible for this decrease.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cells
/
Cell Cycle
/
Cell Culture Techniques
/
Stem Cell Transplantation
/
Intermediate Filament Proteins
/
Nerve Tissue Proteins
/
Neurons
Limits:
Animals
Language:
En
Journal:
Neuroreport
Journal subject:
NEUROLOGIA
Year:
2006
Document type:
Article
Affiliation country:
China