Proliferation and Na+/H+ exchange activation by endothelin in vascular smooth muscle cells.
Am J Hypertens
; 4(9): 776-9, 1991 Sep.
Article
in En
| MEDLINE
| ID: mdl-1657043
ABSTRACT
Initiation and development of proliferative responses to growth factors are often associated to an activation of the Na+/H+ exchange. The present work examined the effect of endothelin (ET-1) on cell proliferation and Na+/H+ exchange in cultured vascular smooth muscle cells. In rat aortic vascular smooth muscle, ET-1 (0.1 to 10 nmol/L) increased the [3H] thymidine uptake in a dose-dependent manner. This effect was enhanced in presence of insulin (0.1 micrograms/mL to 10 micrograms/mL) as a function of concentration. The Na+/H+ exchange, which is a necessary response for mitogenesis, was dose-dependently stimulated by increasing concentrations of ET-1 (1 to 1000 nmol/L) and presented a biphasic response a transient acidification followed by a sustained alkalinization. Alkalinization induced by ET-1 was similar to that obtained by the phorbol 12-myristate 13-acetate (PMA). An inhibitor of protein kinase C, H7, or a long-term pretreatment of cells with PMA for 24 h inhibited the effect of ET-1 and PMA on Na+/H+ exchange. These results confirm that ET-1 could act as a growth factor for vascular smooth muscle cells and suggest that its mode of action depends for a large part to protein kinase C activation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carrier Proteins
/
Endothelins
/
Muscle, Smooth, Vascular
Limits:
Animals
Language:
En
Journal:
Am J Hypertens
Journal subject:
ANGIOLOGIA
Year:
1991
Document type:
Article
Affiliation country:
France