Estradiol prevents release of cytochrome c from mitochondria and inhibits ischemia-induced apoptosis in perfused heart.
Exp Gerontol
; 41(7): 704-8, 2006 Jul.
Article
in En
| MEDLINE
| ID: mdl-16580805
ABSTRACT
The study investigated whether estradiol can prevent release of cytochrome c from mitochondria and induction of apoptosis after 30 and 60 min stop-flow heart ischemia in Langendorff-perfused female rat hearts. Pre-perfusion of hearts with 100 nM 17beta-estradiol prevented the loss of cytochrome c from mitochondria, its accumulation in cytosol, and inhibition of respiration during ischemia. Estradiol strongly reduced activation of caspase-3-like activity and decreased DNA strand breaks in the nuclei of cardiomyocytes (measured by TUNEL staining). The results show that 17beta-estradiol prevents the ischemia-induced release of cytochrome c from mitochondria, subsequent inhibition of mitochondrial respiration, and inhibits caspase activation and apoptosis. Therefore, inhibition of the intrinsic, mitochondria-mediated apoptotic pathway may be one of the mechanisms by which estrogens protect the heart against ischemic damage.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Myocardial Ischemia
/
Apoptosis
/
Cytochromes c
/
Estradiol
/
Mitochondria, Heart
Limits:
Animals
Language:
En
Journal:
Exp Gerontol
Year:
2006
Document type:
Article
Affiliation country:
Lithuania