Dopamine affects cellular immune functions during polymicrobial sepsis.
Intensive Care Med
; 32(5): 731-9, 2006 May.
Article
in En
| MEDLINE
| ID: mdl-16583219
ABSTRACT
OBJECTIVE:
To determine whether infusion of dopamine modulates cellular immune functions and survival during systemic inflammation. DESIGN ANDSETTING:
Randomized animal study, university research laboratory, Level I trauma center.SUBJECTS:
Male NMRI mice.INTERVENTIONS:
Mice were subjected to laparotomy (sham intervention, LAP) or polymicrobial sepsis induced by cecal ligation and puncture (CLP). Mice in each of these conditions received either an intraperitoneal infusion of 0.9% saline (CLP/saline; LAP/saline) or an intraperitoneal infusion of dopamine (1.0 microg/kg/min i.p., CLP/DOP; LAP/DOP). Metabolic data and survival were monitored 24 h and 48 h after onset of sepsis, and animals were terminated 48 h after induction of sepsis to determine splenocyte apoptosis (Annexin V binding capacity), splenocyte proliferation (3H-Thymidine incorporation assay), splenocyte IL-2, IL-6 and IFN-gamma release (ELISA) and leukocyte distribution (WBC; CD3, CD4, CD8, B220, F4/80, NK1.1). MEASUREMENTS ANDRESULTS:
Infusion of dopamine in septic mice increased splenocyte apoptosis and decreased splenocyte proliferation and IL-2 release of septic mice. Furthermore, an inhibitory effect of dopamine infusion on splenocyte proliferation and the release of the TH1-cytokines IL-2 and IFN-gamma was observed in sham operated control mice. These effects were paralleled by a decreased survival of dopamine-treated septic animals (47% vs. 67%). Treatment with DOP did not affect sepsis-induced changes of leukocyte distribution.CONCLUSIONS:
We conclude that dopamine is capable of modulating cellular immune functions in a murine model of sepsis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Dopamine Agents
/
Dopamine
/
Sepsis
/
Immunity, Cellular
Limits:
Animals
Country/Region as subject:
Europa
Language:
En
Journal:
Intensive Care Med
Year:
2006
Document type:
Article
Affiliation country:
Germany
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA