Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing.
J Clin Microbiol
; 44(4): 1209-18, 2006 Apr.
Article
in En
| MEDLINE
| ID: mdl-16597840
ABSTRACT
In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Influenza A virus
/
Gene Expression Regulation, Viral
/
Hemagglutinin Glycoproteins, Influenza Virus
/
Gene Expression Profiling
/
Neuraminidase
Type of study:
Diagnostic_studies
Language:
En
Journal:
J Clin Microbiol
Year:
2006
Document type:
Article
Affiliation country:
United States
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
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US
/
USA