Cytochalasin B-induced superoxide production in polycation-treated neutrophils.
Inflammation
; 15(6): 413-25, 1991 Dec.
Article
in En
| MEDLINE
| ID: mdl-1661706
ABSTRACT
Cytochalasin B alone induces little superoxide production in intact rabbit peritoneal neutrophils. The cytochalasin causes a strong production of superoxide in cells treated with membrane-permeabilizing polycations. Several polycations were able to express the activating effect of cytochalasin B. Especially the poly-L-arginine with a molecular weight of 24,000 proved to be effective. The effectiveness of some polycations is limited because they inactivate the superoxide-generating oxidase system of the neutrophil. Cytochalasin B-induced superoxide production starts at poly-L-arginine concentrations that cause a change of membrane permeability. At the concentrations of cytochalasin B used in our experiments, the binding of [3H]cytochalasin B is not enhanced in poly-L-arginine-treated cells as compared with control cells. Activation of superoxide production by cytochalasin B in polycation-treated neutrophils occurs both in the presence or absence of extracellular Ca2+. When the cells are pretreated with agents that known to interfere with intracellular Ca2+, the subsequent activation is strongly inhibited, suggesting a role for intracellular Ca2+ in cytochalasin B-induced activation. It is suggested that cytochalasin B alone is not able to activate all the steps that eventually result in complete activation of the superoxide-generating oxidase and that membrane perturbation by polycation provides activation of the remaining steps.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyamines
/
Polymers
/
Superoxides
/
Cytochalasin B
/
Neutrophils
Limits:
Animals
Language:
En
Journal:
Inflammation
Year:
1991
Document type:
Article
Affiliation country:
Netherlands