The common bile duct ligation in rat: A relevant in vivo model to study the role of mechanical stress on cell and matrix behaviour.
Histochem Cell Biol
; 126(4): 517-23, 2006 Oct.
Article
in En
| MEDLINE
| ID: mdl-16625364
ABSTRACT
Common bile duct ligation leads to bile accumulation and liver fibrosis. In this model, little attention has been dedicated to the modification of the common bile duct. We have studied by histochemistry and immunohistochemistry, 3 and 5 days after ligation, the connective tissue modifications of the common bile duct wall. After bile duct ligation, compared with normal bile duct, a strong increase of the bile duct diameter, due to bile stasis, and a thickness of the bile duct wall were observed; numerous myofibroblasts expressing alpha-smooth muscle actin appeared in parallel with the detection of many proliferating connective tissue cells. These myofibroblasts secreted very early high amount of elastic fibre components, elastin and fibrillin-1. Elastic fibre increase was also observed close to the epithelial cell layer. Procollagen type III deposition was also induced 3 days after ligation but decreased thereafter, underlining that myofibroblasts modify their synthesis of extracellular matrix components to comply with the request. We show here that common bile duct ligation represents an invaluable model to study myofibroblastic differentiation and extracellular matrix adaptation produced by an acute mechanical stress.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Common Bile Duct
/
Myoblasts, Smooth Muscle
/
Models, Biological
Limits:
Animals
Language:
En
Journal:
Histochem Cell Biol
Journal subject:
CITOLOGIA
/
HISTOCITOQUIMICA
Year:
2006
Document type:
Article
Affiliation country:
France