A highly flexible tRNA acylation method for non-natural polypeptide synthesis.

Murakami, Hiroshi; Ohta, Atsushi; Ashigai, Hiroshi; Suga, Hiroaki

Murakami, Hiroshi; Ohta, Atsushi; Ashigai, Hiroshi; Suga, Hiroaki.

Affiliation

Murakami H; Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904 Tokyo, Japan.

Nat Methods ; 3(5): 357-9, 2006 May.

Article in En | MEDLINE | ID: mdl-16628205

ABSTRACT

ABSTRACT

Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.

Subject(s)

Oligopeptides/chemical synthesis; Protein Engineering/methods; RNA, Transfer/chemistry; Transfer RNA Aminoacylation; Amino Acids/chemistry; Amino Acids/metabolism; RNA, Messenger/chemistry; RNA, Messenger/genetics; RNA, Messenger/metabolism; RNA, Transfer/genetics; RNA, Transfer/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Oligopeptides / RNA, Transfer / Protein Engineering / Transfer RNA Aminoacylation Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2006 Document type: Article Affiliation country: Japan

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