Karyotype analysis of Gossypium arboreum x G. bickii by genome in situ hybridization.
Yi Chuan Xue Bao
; 33(6): 565-72, 2006 Jun.
Article
in En
| MEDLINE
| ID: mdl-16800388
ABSTRACT
By using genome in situ hybridization (GISH) on root somatic chromosomes of allotetraploid derived from the cross Gossypium arboreum x G. bickii with genomic DNA (gDNA) of G. bickii as a probe, two sets of chromosomes, consisting of 26 chromosomes each, were easily distinguished from each other by their distinctive hybridization signals. GISH analysis directly proved that the hybrid G. arboreum x G. bickii is an allotetraploid amphiploid. The karyotype formula of the species was 2n = 4x = 52 = 46m (4sat) + 6sm (4sat). We identified four pairs of satellites with two pairs in each sub-genome. FISH analysis using 45S rDNA as a probe showed that the cross G. arboreum x G. bickii contained 14 NORs. At least five pairs of chromosomes in the G sub-genome showed double hybridization (red and blue) in their long arms, which indicates that chromatin introgression from the A sub-genome had occurred.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyploidy
/
Gossypium
/
Chromosomes, Plant
/
Karyotyping
/
Nucleic Acid Hybridization
Type of study:
Diagnostic_studies
Language:
En
Journal:
Yi Chuan Xue Bao
Year:
2006
Document type:
Article
Affiliation country:
China