Apoptotic effect of ganciclovir on primary effusion lymphoma cells infected with Kaposi's sarcoma-associated herpesvirus.
Nucleosides Nucleotides Nucleic Acids
; 25(4-6): 635-45, 2006.
Article
in En
| MEDLINE
| ID: mdl-16838851
We evaluated the cytotoxic and apoptotic effects of two purine nucleoside analogues, acyclovir (ACV) and ganciclovir (GCV), on lymphoma cells stably harboring Kaposi's sarcoma-associated herpesvirus (KSHV). Colorimetric caspase assay, flow cytometry, and immunoblotting with antibodies against apoptosis-related molecules revealed that GCV has cytotoxic activity toward KSHV-infected primary effusion lymphoma cells, while ACV has weak or little activity. In addition to the GCV-induced cytotoxicity, apoptosis via caspase-7/8, cleavage of poly(ADP-ribose) polymerase, and accumulation of p53 and p21 were induced by GCV treatment. In contrast, neither ACV nor GCV have cytotoxicity- or apoptosis-inducing activities toward uninfected cells.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ganciclovir
/
Apoptosis
/
Herpesvirus 8, Human
/
Lymphoma
Type of study:
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
Nucleosides Nucleotides Nucleic Acids
Journal subject:
BIOQUIMICA
Year:
2006
Document type:
Article
Affiliation country:
Japan
Country of publication:
United States