Heterogeneity of early intermediates in cell-liposome fusion mediated by influenza hemagglutinin.
Biophys J
; 91(9): 3349-58, 2006 Nov 01.
Article
in En
| MEDLINE
| ID: mdl-16905609
ABSTRACT
To explore early intermediates in membrane fusion mediated by influenza virus hemagglutinin (HA) and their dependence on the composition of the target membrane, we studied lipid mixing between HA-expressing cells and liposomes containing phosphatidylcholine (PC) with different hydrocarbon chains. For all tested compositions, our results indicate the existence of at least two types of intermediates, which differ in their lifetimes. The composition of the target membrane affects the stability of fusion intermediates at a stage before lipid mixing. For less fusogenic distearoyl PC-containing liposomes at 4 degrees C, some of the intermediates inactivate, and no intermediates advance to lipid mixing. Fusion intermediates that formed for the more fusogenic dioleoyl PC-containing liposomes did not inactivate and even yielded partial lipid mixing at 4 degrees C. Thus, a more fusogenic target membrane effectively blocks nonproductive release of the conformational energy of HA. Even for the same liposome composition, HA forms two types of fusion intermediates, dissimilar in their stability and propensity to fuse. This diversity of fusion intermediates emphasizes the importance of local membrane composition and local protein concentration in fusion of heterogeneous biological membranes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Hemagglutinin Glycoproteins, Influenza Virus
/
Kidney
/
Liposomes
/
Membrane Fusion
Limits:
Animals
Language:
En
Journal:
Biophys J
Year:
2006
Document type:
Article
Affiliation country:
United States