Activities of 3beta-HSD and aromatase in slices of developing and adult zebra finch brain.
Gen Comp Endocrinol
; 150(1): 26-33, 2007 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-16919626
ABSTRACT
Sex steroids influence the development and function of the songbird brain. Developmentally, the neural circuitry underlying song undergoes masculine differentiation under the influence of estradiol. In adults, estradiol stimulates song behavior and the seasonal growth of song control circuits. There is good reason to believe that these neuroactive estrogens are synthesized in the brain. At all ages, estrogens could act at the lateral ventricle, during migration, or where song nuclei exist or will form. We investigated the activity of two critical steroidogenic enzymes, 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD) and aromatase, using a slice culture system. Sagittal brain slices were collected from juvenile (posthatch day 20) and adult zebra finches containing either the lateral ventricle, where neurons are born, or the telencephalic song nuclei HVC and RA. The slices were incubated with (3)H-dehydroepiandrosterone or (3)H-androstenedione. Activity was determined by isolating certain products of 3beta-HSD (5alpha-androstanedione, 5beta-androstanedione, estrone, and estradiol) and aromatase (estrone and estradiol). Activities of both 3beta-HSD and aromatase were detected in all slices and were confirmed using specific enzyme inhibitors. We found no significant difference in activity between adult males and females in either region for either enzyme. Juvenile female slices containing the lateral ventricle, however, showed greater levels of 3beta-HSD activity than did similar slices from age-matched males. Determination of the activity of these critical steroidogenic enzymes in slice culture has implications for the role of neurosteroids in brain development.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Progesterone Reductase
/
Steroid Isomerases
/
Aromatase
/
Lateral Ventricles
/
Finches
/
High Vocal Center
/
Multienzyme Complexes
Limits:
Animals
Language:
En
Journal:
Gen Comp Endocrinol
Year:
2007
Document type:
Article
Affiliation country:
United States