Detection, characterization and identification of crucifer phytoalexins using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.
J Chromatogr A
; 1133(1-2): 172-83, 2006 Nov 10.
Article
in En
| MEDLINE
| ID: mdl-16949596
ABSTRACT
We have analyzed 23 crucifer phytoalexins (e.g. brassinin, dioxibrassinin, cyclobrassinin, brassicanals A and C) by HPLC with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) using both negative and positive ion modes. Positive ion mode ESI-MS appeared more sensitive than negative ion mode ESI-MS in detecting this group of compounds. A new HPLC separation method, new LC-MS and LC-MS(2) data and proposed fragmentation pathways, LC retention times, and UV spectra for selected compounds are reported.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Terpenes
/
Chromatography, High Pressure Liquid
/
Brassicaceae
/
Spectrometry, Mass, Electrospray Ionization
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
J Chromatogr A
Year:
2006
Document type:
Article
Affiliation country:
Canada
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS