Detection, characterization and identification of crucifer phytoalexins using high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry.

Pedras, M Soledade C; Adio, Adewale M; Suchy, Mojmir; Okinyo, Denis P O; Zheng, Qing-An; Jha, Mukund; Sarwar, Mohammed G

Pedras, M Soledade C; Adio, Adewale M; Suchy, Mojmir; Okinyo, Denis P O; Zheng, Qing-An; Jha, Mukund; Sarwar, Mohammed G.

Affiliation

Pedras MS; Department of Chemistry, University of Saskatchewan, 110 Science Place, Saskatoon, SK S7N 5C9, Canada. s.pedras@usask.ca

J Chromatogr A ; 1133(1-2): 172-83, 2006 Nov 10.

Article in En | MEDLINE | ID: mdl-16949596

ABSTRACT

ABSTRACT

We have analyzed 23 crucifer phytoalexins (e.g. brassinin, dioxibrassinin, cyclobrassinin, brassicanals A and C) by HPLC with diode array detection and electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS) using both negative and positive ion modes. Positive ion mode ESI-MS appeared more sensitive than negative ion mode ESI-MS in detecting this group of compounds. A new HPLC separation method, new LC-MS and LC-MS(2) data and proposed fragmentation pathways, LC retention times, and UV spectra for selected compounds are reported.

Subject(s)

Brassicaceae/chemistry; Chromatography, High Pressure Liquid/methods; Spectrometry, Mass, Electrospray Ionization/methods; Terpenes/analysis; Molecular Structure; Reproducibility of Results; Sesquiterpenes; Terpenes/chemistry; Phytoalexins

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Collection: 01-internacional Database: MEDLINE Main subject: Terpenes / Chromatography, High Pressure Liquid / Brassicaceae / Spectrometry, Mass, Electrospray Ionization Type of study: Diagnostic_studies / Prognostic_studies Language: En Journal: J Chromatogr A Year: 2006 Document type: Article Affiliation country: Canada Publication country: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS

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