Towards many colors in FISH on 3D-preserved interphase nuclei.
Cytogenet Genome Res
; 114(3-4): 367-78, 2006.
Article
in En
| MEDLINE
| ID: mdl-16954680
ABSTRACT
The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + wide-field microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome - one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Nucleus
/
In Situ Hybridization, Fluorescence
/
Interphase
Limits:
Humans
Language:
En
Journal:
Cytogenet Genome Res
Journal subject:
GENETICA
Year:
2006
Document type:
Article
Affiliation country:
Germany