A CD34(+) human cell line model of myeloid dendritic cell differentiation: evidence for a CD14(+)CD11b(+) Langerhans cell precursor.
J Leukoc Biol
; 80(6): 1337-44, 2006 Dec.
Article
in En
| MEDLINE
| ID: mdl-16959899
ABSTRACT
The study of early events in dendritic cell (DC) differentiation is hampered by the lack of homogeneous primary cell systems that allow the study of cytokine-driven, transitional DC differentiation steps. The CD34(+) acute myeloid leukemia cell line MUTZ-3 displays a unique ability to differentiate into interstitial DC (IDC) and Langerhans cells (LC) in a cytokine-dependent manner. Phenotypic characterization revealed MUTZ-3 to consist of three distinct subpopulations. Small CD34(+)CD14(-)CD11b(-) progenitors constitute the proliferative compartment of the cell line with the ability to differentiate through a CD34(-)CD14(-)CD11b(+) stage to ultimately give rise to a morphologically large, nonproliferating CD14(+)CD11b(hi) progeny. These CD14(+)CD11b(hi) cells were identified as common, immediate myeloid DC precursors with the ability to differentiate into LC and IDC, exhibiting characteristic and mutually exclusive expression of Langerin and DC-specific ICAM-grabbing nonintegrin, respectively. The identity of the MUTZ-3-derived LC subset was confirmed further by the presence of Birbeck granules. We conclude that the MUTZ-3 cell line provides a ready and continuous supply of common myeloid precursors, which should facilitate further study of the ontogeny of myeloid DC lineages.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Differentiation
/
Langerhans Cells
/
Models, Immunological
/
Antigens, CD34
/
Lipopolysaccharide Receptors
/
CD11b Antigen
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Leukoc Biol
Year:
2006
Document type:
Article
Affiliation country:
Netherlands