Selective identification of HLA-DP4 binding T cell epitopes encoded by the MAGE-A gene family.
Cancer Immunol Immunother
; 56(6): 807-18, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-16988823
ABSTRACT
Because of the high frequency of HLA-DP4 in the Caucasian population, we have selectively delineated HLA-DP4 restricted T cell epitopes in the MAGE-A tumor antigens. We identified 12 good binders to HLA-DP4 and investigated the capacity of the seven best binders to induce in vitro specific CD4+ T cell lines from HLA-DP4 healthy donors. We found that the MAGE-A1 90-104 peptide exhibited a high and constant frequency of CD4+ T cell precursors in all the six tested donors. The MAGE-A1 268-282 peptide was found immunogenic in only two donors but with a high precursor frequency. The MAGE-A12 127-141 peptide was T cell stimulating in six different donors and induced fewer T cell lines. The peptide-specific T cell lines were stimulated by DC loaded with the lysates of cells transfected with MAGE-A1 or MAGE-A12, or loaded with the recombinant protein. We also show that the immunoreactivity of CD4+ T cell epitopes restricted to the same HLA II molecule may vary from one individual to another, as a result of inter-individual variations in the CD4+ T cell repertoire.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
HLA-DP Antigens
/
Epitopes, T-Lymphocyte
/
Antigens, Neoplasm
/
Neoplasm Proteins
Type of study:
Diagnostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Cancer Immunol Immunother
Journal subject:
ALERGIA E IMUNOLOGIA
/
NEOPLASIAS
/
TERAPEUTICA
Year:
2007
Document type:
Article
Affiliation country:
France