Optimization of inactivation of endospores of Bacillus cereus by antimicrobial lipopeptides from Bacillus subtilis fmbj strains using a response surface method.
Appl Microbiol Biotechnol
; 74(2): 454-61, 2007 Feb.
Article
in En
| MEDLINE
| ID: mdl-17043814
ABSTRACT
Bacillus subtilis fmbj can produce a lipopeptide antimicrobial substance, the main components of which are surfactin and fengycin. In this paper, the sensitivity of Bacillus cereus to antimicrobial lipopeptides from B. subtilis fmbj was observed, and the effect of the microstructure of antimicrobial lipopeptide on spores of B. cereus was investigated. At the same time, the optimization of the inactivation of antimicrobial lipopeptides to spores of B. cereus by a response surface methodology was studied. Results showed that B. cereus had high sensitivity to it, whose minimal inhibitory concentration was 156.25 microg/ml. It could result in the death of spores by destroying the structure of resting spores and sprouting spores, as was observed by transmission electron microscopy. The optimization result indicated that spores of B. cereus could be inactivated by 2 orders of magnitude when the temperature was 29.6 degrees C, the action time was 7.6 h, and the concentration was 3.46 mg.ml(-1).
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spores, Bacterial
/
Bacillus cereus
/
Microbial Sensitivity Tests
/
Lipoproteins
/
Anti-Bacterial Agents
Type of study:
Diagnostic_studies
/
Prognostic_studies
Language:
En
Journal:
Appl Microbiol Biotechnol
Year:
2007
Document type:
Article
Publication country:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY