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[Influence of meliorated AS-PCR primer for ABO genotyping].
Wang, Xin-Jie; Diao, Li-Jiang; Feng, Jian-Zhong; Lu, Gui-Ping.
Affiliation
  • Wang XJ; Weifang Public Security Bureau, Weifang 261061, China.
Fa Yi Xue Za Zhi ; 22(4): 275-6, 2006 Aug 15.
Article in Zh | MEDLINE | ID: mdl-17080666
ABSTRACT

OBJECTIVE:

To improve the correct rate of ABO genotyping by meliorating AS-PCR primer.

METHODS:

The primer P1 was changed into primer P1' by substituting the fifth base G for C of 3' end and the ABO genotyping results of primer P1 and P1' was compared and analysed.

RESULTS:

The non-specific product of OO typing is reducing and the wrong genotyping of OO and AO was avoided by meliorating AS-PCR primer.

CONCLUSION:

The rates of wrong ABO genotyping results could be effectively reduced by using altering primer P1'.
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Collection: 01-internacional Database: MEDLINE Main subject: ABO Blood-Group System / Polymerase Chain Reaction / DNA Primers / Alleles Type of study: Diagnostic_studies Limits: Humans Language: Zh Journal: Fa Yi Xue Za Zhi Journal subject: JURISPRUDENCIA Year: 2006 Document type: Article Affiliation country: China
Search on Google
Add to My VHL
Print
XML
PubMed Links

Collection: 01-internacional Database: MEDLINE Main subject: ABO Blood-Group System / Polymerase Chain Reaction / DNA Primers / Alleles Type of study: Diagnostic_studies Limits: Humans Language: Zh Journal: Fa Yi Xue Za Zhi Journal subject: JURISPRUDENCIA Year: 2006 Document type: Article Affiliation country: China