Assay development and case history of a 32K-biased library high-content MK2-EGFP translocation screen to identify p38 mitogen-activated protein kinase inhibitors on the ArrayScan 3.1 imaging platform.

Trask, Oscar J; Baker, Audrey; Williams, Rhonda Gates; Nickischer, Debra; Kandasamy, Ramani; Laethem, Carmen; Johnston, Patricia A; Johnston, Paul A

Trask, Oscar J; Baker, Audrey; Williams, Rhonda Gates; Nickischer, Debra; Kandasamy, Ramani; Laethem, Carmen; Johnston, Patricia A; Johnston, Paul A.

Affiliation

Trask OJ; Sphinx RTP Laboratories, Eli Lilly and Company, Research Triangle Park, NC, USA.

Methods Enzymol ; 414: 419-39, 2006.

Article in En | MEDLINE | ID: mdl-17110205

Subject(s)

Combinatorial Chemistry Techniques/instrumentation; Green Fluorescent Proteins/chemistry; Protein Kinases/chemistry; p38 Mitogen-Activated Protein Kinases/chemistry; Biological Assay/methods; Cell Adhesion; Cell Nucleus/metabolism; Combinatorial Chemistry Techniques/methods; Cytoplasm/metabolism; Gene Library; Humans; Inhibitory Concentration 50; Intracellular Signaling Peptides and Proteins; MAP Kinase Signaling System; Phosphorylation; Protein Serine-Threonine Kinases; Protein Transport; Translocation, Genetic

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Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinases / Combinatorial Chemistry Techniques / P38 Mitogen-Activated Protein Kinases / Green Fluorescent Proteins Type of study: Prognostic_studies Limits: Humans Language: En Journal: Methods Enzymol Year: 2006 Document type: Article Affiliation country: United States Country of publication: United States

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