The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells.
Cell Immunol
; 242(2): 110-7, 2006 Aug.
Article
in En
| MEDLINE
| ID: mdl-17116296
ABSTRACT
We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4(+) T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4(+) T cells that secreted elevated levels of IFN-gamma, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to naïve recipients using CPAF-specific CD4(+) T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4(+) T cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Endopeptidases
/
Bacterial Vaccines
/
CD4-Positive T-Lymphocytes
/
Chlamydia
Limits:
Animals
Language:
En
Journal:
Cell Immunol
Year:
2006
Document type:
Article
Affiliation country:
United States