Rat paw oedema and mast cell degranulation caused by two phospholipase A2 enzymes isolated from Trimeresurus mucrosquamatus venom.
J Pharm Pharmacol
; 42(12): 846-50, 1990 Dec.
Article
in En
| MEDLINE
| ID: mdl-1713267
ABSTRACT
Two phospholipase A2 (PLA2) enzymes, TMVPLA2 I and TMVPLA2 II, isolated from Trimeresurus mucrosquamatus venom (TMV) induce rat hind-paw oedema in a dose-dependent manner. This response is suppressed by pretreatment with diphenhydramine, methysergide or compound 48/80, which reduces tissue histamine content. In isolated mast cells, TMVPLA2 I and TMVPLA2 II cause concentration-, time- and calcium-dependent release of histamine and beta-glucuronidase. This effect is inhibited by disodium cromoglycate, mepacrine, nordihydroguaiaretic acid, piriprost and BW 755C, but not by aspirin or indomethacin. These observations indicate that the mast cell plays a predominant role in TMVPLA2 I- and TMVPLA2 II-induced paw oedema, and that venom PLA2 enzyme needs an intact lipoxygenase pathway to induce mast cell degranulation.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipases
/
Phospholipases A
/
Snake Venoms
/
Crotalid Venoms
/
Edema
/
Mast Cells
Limits:
Animals
Language:
En
Journal:
J Pharm Pharmacol
Year:
1990
Document type:
Article
Affiliation country:
Taiwan