Functional reconstitution of an archaeal splicing endonuclease in vitro.
Nucleic Acids Symp Ser (Oxf)
; (49): 103-4, 2005.
Article
in En
| MEDLINE
| ID: mdl-17150654
ABSTRACT
Sulfolobus tokodaii strain 7 is one of archaea whose entire genome has been sequenced. The genome sequence revealed that it possesses two open reading frames (ORFs) that are homologous to endA, a protein responsible for splicing endonuclease activity in archaea. Interestingly, one of these two ORFs lacks a putative catalytic amino acid residue for the nuclease activity. To investigate their functions, the two ORFs were individually expressed in E. coli, partially purified, and tested for their nuclease activities in vitro. Using in vitro transcribed tRNA precursors as substrates, we found that the two ORF products are concurrently required to cleave exon-intron junctions. Our finding implies that the splicing endonuclease of the organism is a multi-subunit complex composed of the two ORF products.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sulfolobus
/
Archaeal Proteins
/
Endoribonucleases
Language:
En
Journal:
Nucleic Acids Symp Ser (Oxf)
Year:
2005
Document type:
Article
Affiliation country:
Japan