Construction of a stable expression vector for Leifsonia xyli subsp. cynodontis and its application in studying the effect of the bacterium as an endophytic bacterium in rice.
FEMS Microbiol Lett
; 267(2): 176-83, 2007 Feb.
Article
in En
| MEDLINE
| ID: mdl-17166228
ABSTRACT
To study the possibility of utilizing genetically engineered Leifsonia xyli subsp. cynodontis (Lxc) as an endophytic bacterium in rice, we constructed an Escherichia coli-Lxc shuttle vector, pLGUS, containing a beta-glucuronidase reporter gene, which was stable both in vitro and in vivo. Lxc grows and expresses the beta-glucuronidase reporter gene in all parts of rice, except for seed. A 2-year field study using three rice varieties from China showed that Lxc inoculation did not have a negative effect on the growth and yield of any of these varieties. Therefore, Lxc has the potential to be used as a benign endophyte for the expression of foreign genes in rice.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oryza
/
Actinomycetales
/
Genetic Vectors
Language:
En
Journal:
FEMS Microbiol Lett
Year:
2007
Document type:
Article
Affiliation country:
China