Molecular cloning of a lectin cDNA from Alocasia macrorrhiza and prediction of its characteristics.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
; 32(6): 634-42, 2006 Dec.
Article
in En
| MEDLINE
| ID: mdl-17167199
ABSTRACT
The cDNA of Alocasia macrorrhiza lectin (aml, GenBank accession number DQ340864) was cloned by RACE-PCR and its characteristics were predicted by various bioinformatics tools. GSPs (Gene Specific Primers) were designed according to the conserved regions of the genes encoded for lectins and similar proteins from the same family Araceae. Total RNAs were extracted from the tubers of A macrorrhiza by Qiagen RNeasy mini kit. The 3'- and 5'-RACE-PCRs were performed with the isolated total RNAs by SMART(TM)RACE cDNA amplification kit from BD Biosciences Clontech Company, respectively. The purified PCR products were ligated with pMD 18-T vector, and the confirmed clones were sequenced. The full-length cDNA of aml was obtained by combination of 3'- and 5'-end sequences, and was then confirmed by full-length 3'-RACE-PCR. The aml cDNA is 1 124 bp long. The deduced amino acid length of AML lectin is 270 aa. Its relative molecular weight is 29.7 kD. The results of homologous analysis showed a high similarity between AML and other mannose-binding lectins and similar proteins from Araceae family. Two typical B-lectin domains and three mannose- binding motifs were found in the sequence of AML. With all these taken together, it can be concluded that this newly cloned aml cDNA encodes for a mannose-binding lectin.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Complementary
/
Alocasia
/
Plant Lectins
Type of study:
Prognostic_studies
/
Risk_factors_studies
Language:
En
Journal:
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao
Journal subject:
BIOLOGIA MOLECULAR
/
BOTANICA
Year:
2006
Document type:
Article
Affiliation country:
China