Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples.

Diederen, Bram M W; de Jong, Caroline M A; Marmouk, Faïçal; Kluytmans, Jan A J W; Peeters, Marcel F; Van der Zee, Anneke

Diederen, Bram M W; de Jong, Caroline M A; Marmouk, Faïçal; Kluytmans, Jan A J W; Peeters, Marcel F; Van der Zee, Anneke.

Affiliation

Diederen BMW; Laboratory of Medical Microbiology and Immunology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands.
de Jong CMA; Laboratory of Medical Microbiology and Immunology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands.
Marmouk F; Laboratory of Medical Microbiology and Immunology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands.
Kluytmans JAJW; Laboratory of Microbiology and Infection Control, Amphia Hospital, PO Box 90158, 4800 RK Breda, The Netherlands.
Peeters MF; Laboratory of Medical Microbiology and Immunology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands.
Van der Zee A; Laboratory of Medical Microbiology and Immunology, St Elisabeth Hospital, PO Box 747, 5000 AS Tilburg, The Netherlands.

J Med Microbiol ; 56(Pt 1): 94-101, 2007 Jan.

Article in En | MEDLINE | ID: mdl-17172523

Subject(s)

DNA, Bacterial/analysis; Legionella pneumophila/genetics; Legionnaires' Disease/diagnosis; Polymerase Chain Reaction/methods; Adult; Aged; Bacterial Proteins/genetics; DNA, Bacterial/blood; DNA, Bacterial/genetics; Female; Humans; Legionnaires' Disease/blood; Legionnaires' Disease/microbiology; Male; Middle Aged; Molecular Diagnostic Techniques/methods; Molecular Diagnostic Techniques/standards; Peptidylprolyl Isomerase/genetics; Polymerase Chain Reaction/standards; RNA, Ribosomal, 16S/genetics; RNA, Ribosomal, 5S/genetics; Reproducibility of Results; Sensitivity and Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / Legionnaires' Disease / Polymerase Chain Reaction / Legionella pneumophila Type of study: Diagnostic_studies / Evaluation_studies / Screening_studies Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: En Journal: J Med Microbiol Year: 2007 Document type: Article Affiliation country: Netherlands

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