Structural polypeptides of type II avian adenoviruses analyzed by monoclonal and polyclonal antibodies.
Avian Dis
; 35(3): 572-8, 1991.
Article
in En
| MEDLINE
| ID: mdl-1719952
ABSTRACT
Polypeptides of hemorrhagic enteritis virus (HEV) of turkeys and marble spleen disease virus (MSDV) of pheasants were analyzed by immune precipitation and immunoblot assays. A total of 11 polypeptides ranging in molecular weight from 14,000 to 97,000 were detected in lysates of HEV-infected turkey cells analyzed by immunoblot assay using a polyclonal antibody against HEV. Identical patterns were observed with preparations of MSDV. Five monoclonal antibodies (MAbs) against HEV were chosen based on their virus neutralization activity and used for identification of neutralizing epitopes of these two viruses. Three MAbs precipitated a single 97,000-molecular-weight hexon polypeptide in an immune precipitation assay.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Viral Proteins
/
Aviadenovirus
/
Immune Sera
/
Antibodies, Monoclonal
Limits:
Animals
Language:
En
Journal:
Avian Dis
Year:
1991
Document type:
Article