Isolation of a gene that is involved in Campylobacter jejuni 81116 cytotoxin activation.
FEMS Immunol Med Microbiol
; 49(1): 133-40, 2007 Feb.
Article
in En
| MEDLINE
| ID: mdl-17266719
ABSTRACT
Cytotoxin fractions were isolated from Campylobacter jejuni 81116 and semi-purified by size-exclusion liquid chromatography. The fraction showing the strongest toxicity was injected into mice to produce antiserum. The antiserum was used to screen a C. jejuni 81116 cosmid library. Nine genes were identified in overlapping cosmid inserts that induced reactivity with the antiserum. One of these genes showed high similarity to a periplasmic protein of unknown function and its isogenic mutant showed decreased toxicity compared to the C. jejuni 81116 wild type. This gene contains a Gram-negative bacterial RTX toxin-activating protein C signature, which suggests it may play a role in C. jejuni 81116 cytotoxin activation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cosmids
/
Campylobacter jejuni
/
Cytotoxins
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
FEMS Immunol Med Microbiol
Journal subject:
ALERGIA E IMUNOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
MICROBIOLOGIA
Year:
2007
Document type:
Article
Affiliation country:
Australia