Mitochondrial OXPHOS functions in R1H rhabdomyosarcoma and skeletal muscles of the rat.
Neurochem Res
; 32(6): 973-80, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17273927
ABSTRACT
The aim of the study was to determinate mitochondrial oxidative phosphorylation (OXPHOS) functions in rat rhabdomyosarcoma R1H (R1H) and rat skeletal muscles. For that purpose skinned fiber technique and multiple substrate inhibitor titration were adapted to tumor samples. In our animal tumor model (R1H) functional abnormalities of OXPHOS were found compared to skeletal muscles. In R1H the state 3 respiration of pyruvate + malate was decreased 0.56 +/- 0.28 nmol O(2)/mg/min versus 2.32 +/- 1.19 nmol O(2)/mg/min, P < 0.001, whereas the state 3 respiration of succinate + rotenone was increased 36 +/- 14% versus 19 +/- 11%, P < 0.001. In R1H the rotenone-insensitive respiration reached higher levels than the antimycin A-insensitive respiration, whereas in normal muscles the converse was observed. Additionally, the obvious difference between the CAT- and the antimycin A-independent respiration indicates an increased part of leak respiration in R1H. By now, the high feasibility of these techniques is appreciated for the investigation of muscles and prospectively for tumors, too.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxidative Phosphorylation
/
Rhabdomyosarcoma
/
Muscle, Skeletal
/
Mitochondria, Muscle
Limits:
Animals
Language:
En
Journal:
Neurochem Res
Year:
2007
Document type:
Article
Affiliation country:
Germany
Country of publication:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA