How to determine local elastic properties of lipid bilayer membranes from atomic-force-microscope measurements: a theoretical analysis.
Phys Rev E Stat Nonlin Soft Matter Phys
; 74(6 Pt 1): 061914, 2006 Dec.
Article
in En
| MEDLINE
| ID: mdl-17280103
ABSTRACT
Measurements with an atomic force microscope (AFM) offer a direct way to probe elastic properties of lipid bilayer membranes locally provided the underlying stress-strain relation is known, material parameters such as surface tension or bending rigidity may be deduced. In a recent experiment a pore-spanning membrane was poked with an AFM tip, yielding a linear behavior of the force-indentation curves. A theoretical model for this case is presented here which describes these curves in the framework of Helfrich theory. The linear behavior of the measurements is reproduced if one neglects the influence of adhesion between tip and membrane. Including it via an adhesion balance changes the situation significantly force-distance curves cease to be linear, hysteresis and nonzero detachment forces can show up. The characteristics of this rich scenario are discussed in detail in this paper.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Models, Molecular
/
Microscopy, Atomic Force
/
Lipid Bilayers
/
Liposomes
/
Membrane Fluidity
/
Micromanipulation
/
Models, Chemical
Type of study:
Prognostic_studies
Language:
En
Journal:
Phys Rev E Stat Nonlin Soft Matter Phys
Journal subject:
BIOFISICA
/
FISIOLOGIA
Year:
2006
Document type:
Article
Affiliation country:
Iran