[Kappa-opioid receptor mediates the cardioprotective effect of ischemic postconditioning].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 36(1): 41-7, 2007 01.
Article
in Zh
| MEDLINE
| ID: mdl-17290490
ABSTRACT
OBJECTIVE:
To investigate the effect of kappa-Opioid receptors in the cardioprotection elicited by ischemic postconditioning and the underlying mechanism.METHODS:
The isolated perfused hearts of male Sprague-Dawley rats were subjected to 30 min of global ischemia followed by 120 min of reperfusion. formazan content of myocardium was measured spectrophotometrically, and the level of lactate dehydrogenase (LDH) in the coronary effluent was also measured. In isolated ventricular myocytes hypoxia postconditioning was achieved by 3 cycles of 5 min reoxygenation/5 min hypoxia starting at the beginning of reoxygenation, and cell viability was measured.RESULT:
In the Langendorff perfused rat heart model, ischemic postconditioning (6 cycles of 10 s reperfusion/10 s global ischemia starting at the beginning of reperfusion) increased formazan content, reduced LDH release, improved the recovery of the left ventricular developed pressure, maximal rise/fall rate of left ventricular pressure, left ventricular end-diastolic pressure and rate pressure product (left ventricular developed pressure multiplied by heart rate), attenuated the decrease of coronary flow during reperfusion and increased the isolated cell viability. Pretreatment with nor-BNI, an antagonist of kappa-Opioid receptors and mitoK(ATP) blocker 5-HD attenuated the effect of ischemic/hypoxic postconditioning.CONCLUSION:
Postconditioning may protect myocardium against ischemia/reperfusion injury via activating kappa-Opioid receptors and mitoK(KATP).
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Myocardial Reperfusion Injury
/
Potassium Channels
/
Receptors, Opioid, kappa
/
Ischemic Preconditioning, Myocardial
Limits:
Animals
Language:
Zh
Journal:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Journal subject:
MEDICINA
Year:
2007
Document type:
Article
Affiliation country:
China