Neurotoxic activity of Gln49 phospholipase A(2) from Gloydius ussuriensis snake venom.
J Appl Toxicol
; 27(5): 447-52, 2007.
Article
in En
| MEDLINE
| ID: mdl-17299814
ABSTRACT
A novel neurotoxic protein phospholipase A(2) (PLA(2)), molecular weight 13 881.83 Da, has been isolated from snake venom of Gloydius ussuriensis, named as Gln49-PLA(2), which shows weak lethal toxic, myotoxic and apparent anticoagulant activity, but lacks phospholipase activity. The Gln49-PLA(2) obviously induced an increase of the pain threshold in intoxicated 615 mice compared with the control group, suggesting it is a neurotoxin. Hot-plate tests also showed that its analgesic activity was dose-dependent, and naloxone antagonized the analgesic effect, implying the mechanism of action of Gln49-sPLA(2) is correlated with opioid receptors. Electrophysiology studies revealed decreases in the action potential and the nerve conduction velocity in isolated hoptoad (Bufo bufo gargarizans Cantor) sciatic nerve, indicating Gln49-PLA(2) most probably had effects on ion channels.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipases A
/
Crotalid Venoms
/
Neurotoxins
Limits:
Animals
Language:
En
Journal:
J Appl Toxicol
Year:
2007
Document type:
Article