The aryl hydrocarbon receptor agonist 3,3',4,4',5-pentachlorobiphenyl induces distinct patterns of gene expression between hepatoma and glioma cells: chromatin remodeling as a mechanism for selective effects.
Neurotoxicology
; 28(3): 594-612, 2007 May.
Article
in En
| MEDLINE
| ID: mdl-17316808
Genome-wide oligonucleotide DNA microarrays and real time RT-PCR were used to assess differential gene expression in rat glioma and hepatoma cell lines after exposure to the aryl hydrocarbon receptor (AhR) agonist 3,3',4,4',5-pentachlorobiphenyl (penta-CB). Under maximal inducing concentrations for cytochrome P450 1A1 (CYP1A1) in H4IIE rat hepatoma cells, both H4IIE and C6 rat glioma cells were exposed to sub-micromolar concentrations of penta-CB for 24h. Differential gene expression for approximately 28,000 gene probes were computationally analyzed and compared. As expected, penta-CB potently activated CYP1A1/2 transcription in liver-derived H4IIE hepatoma cells yet did not do so in brain-derived C6 glioma cells. Additionally, we show that penta-CB causes: (1) distinct patterns of gene expression between tumor cells derived from liver or brain; (2) robust transcriptional activation of select C6 glioma gene ontologies; (3) over-expression of H4IIE hepatoma genes associated with tumor progression in liver; (4) greater than 100-fold over-expression of C6 glioma genes associated with protein processing and programmed cell death and/or metastasis; (5) tissue-selective histone deacetylase inhibition in C6 glioma, but not H4IIE hepatoma cells as signaled by galectin-1 over-expression.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Neoplasms
/
Chromatin
/
Gene Expression Regulation, Neoplastic
/
Polychlorinated Biphenyls
/
Receptors, Aryl Hydrocarbon
/
Glioma
/
Liver Neoplasms
/
Liver Neoplasms, Experimental
Limits:
Animals
Language:
En
Journal:
Neurotoxicology
Year:
2007
Document type:
Article
Affiliation country:
United States
Country of publication:
Netherlands