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The aryl hydrocarbon receptor agonist 3,3',4,4',5-pentachlorobiphenyl induces distinct patterns of gene expression between hepatoma and glioma cells: chromatin remodeling as a mechanism for selective effects.
Maier, Mark S V; Legare, Marie E; Hanneman, William H.
Affiliation
  • Maier MS; Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523-1680, USA.
Neurotoxicology ; 28(3): 594-612, 2007 May.
Article in En | MEDLINE | ID: mdl-17316808
Genome-wide oligonucleotide DNA microarrays and real time RT-PCR were used to assess differential gene expression in rat glioma and hepatoma cell lines after exposure to the aryl hydrocarbon receptor (AhR) agonist 3,3',4,4',5-pentachlorobiphenyl (penta-CB). Under maximal inducing concentrations for cytochrome P450 1A1 (CYP1A1) in H4IIE rat hepatoma cells, both H4IIE and C6 rat glioma cells were exposed to sub-micromolar concentrations of penta-CB for 24h. Differential gene expression for approximately 28,000 gene probes were computationally analyzed and compared. As expected, penta-CB potently activated CYP1A1/2 transcription in liver-derived H4IIE hepatoma cells yet did not do so in brain-derived C6 glioma cells. Additionally, we show that penta-CB causes: (1) distinct patterns of gene expression between tumor cells derived from liver or brain; (2) robust transcriptional activation of select C6 glioma gene ontologies; (3) over-expression of H4IIE hepatoma genes associated with tumor progression in liver; (4) greater than 100-fold over-expression of C6 glioma genes associated with protein processing and programmed cell death and/or metastasis; (5) tissue-selective histone deacetylase inhibition in C6 glioma, but not H4IIE hepatoma cells as signaled by galectin-1 over-expression.
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Collection: 01-internacional Database: MEDLINE Main subject: Brain Neoplasms / Chromatin / Gene Expression Regulation, Neoplastic / Polychlorinated Biphenyls / Receptors, Aryl Hydrocarbon / Glioma / Liver Neoplasms / Liver Neoplasms, Experimental Limits: Animals Language: En Journal: Neurotoxicology Year: 2007 Document type: Article Affiliation country: United States Country of publication: Netherlands
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Collection: 01-internacional Database: MEDLINE Main subject: Brain Neoplasms / Chromatin / Gene Expression Regulation, Neoplastic / Polychlorinated Biphenyls / Receptors, Aryl Hydrocarbon / Glioma / Liver Neoplasms / Liver Neoplasms, Experimental Limits: Animals Language: En Journal: Neurotoxicology Year: 2007 Document type: Article Affiliation country: United States Country of publication: Netherlands