A rapid, homogeneous, fluorescence polarization binding assay for peroxisome proliferator-activated receptors alpha and gamma using a fluorescein-tagged dual PPARalpha/gamma activator.

Seethala, Ramakrishna; Golla, Rajasree; Ma, Zhengping; Zhang, Hao; O'Malley, Kevin; Lippy, Jonathan; Cheng, Lin; Mookhtiar, Kasim; Farrelly, Dennis; Zhang, Litao; Hariharan, Narayanan; Cheng, Peter T W

Seethala, Ramakrishna; Golla, Rajasree; Ma, Zhengping; Zhang, Hao; O'Malley, Kevin; Lippy, Jonathan; Cheng, Lin; Mookhtiar, Kasim; Farrelly, Dennis; Zhang, Litao; Hariharan, Narayanan; Cheng, Peter T W.

Affiliation

Seethala R; Drug Discovery Division, Pharmaceutical Research Institute, Bristol-Myers Squibb, Princeton, NJ 08543, USA. ramakrishna.seethala@bms.com <ramakrishna.seethala@bms.com>

Anal Biochem ; 363(2): 263-74, 2007 Apr 15.

Article in En | MEDLINE | ID: mdl-17335769

Subject(s)

Fluorescence Polarization/methods; Ligands; PPAR alpha/metabolism; PPAR gamma/metabolism; Chromatography, Gel; Dimethyl Sulfoxide/chemistry; Humans; Kinetics; Models, Biological; Molecular Structure; PPAR alpha/agonists; PPAR alpha/genetics; PPAR gamma/agonists; PPAR gamma/genetics; Protein Binding; Reproducibility of Results

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Collection: 01-internacional Database: MEDLINE Main subject: PPAR alpha / PPAR gamma / Fluorescence Polarization / Ligands Type of study: Prognostic_studies Limits: Humans Language: En Journal: Anal Biochem Year: 2007 Document type: Article Country of publication: United States

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