Identification of disease-associated proteins by proteomic approach in ankylosing spondylitis.
Biochem Biophys Res Commun
; 357(2): 531-6, 2007 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-17434140
ABSTRACT
Ankylosing spondylitis (AS) is a chronic systemic inflammatory disorder of the axial skeleton and shows significant inherited susceptibility. Auto-immune responses have been traditionally considered as a putative pathogenetic event in AS. However, no consistent self-antigen has been identified to responsible for the disorders in AS to this day. In this study, serum protein profiles of AS patients and healthy controls from a large Chinese AS family were investigated by two dimensional electrophoresis analysis. A group of four highly expressed protein spots was observed in all AS patients' profiles and subsequently identified as isoforms of haptoglobin precursor (pre-Hp) by ESI-Q-TOF MS/MS. Increased expression of haptoglobin precursor were also observed in sera of sporadic AS patients. Moreover, bioinformatics analysis revealed epitopes derived from haptoglobin precursor with high affinity binding to HLA-B( *)2705, a primary subtype associated with AS. These results indicate that pre-Hp may be involved in the pathogenesis of AS.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Spondylitis, Ankylosing
/
Haptoglobins
/
Proteome
Type of study:
Diagnostic_studies
/
Risk_factors_studies
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Biochem Biophys Res Commun
Year:
2007
Document type:
Article
Affiliation country:
China