Cryopreservation of mouse embryoid bodies.
Cryobiology
; 54(3): 290-3, 2007 Jun.
Article
in En
| MEDLINE
| ID: mdl-17442298
ABSTRACT
Cryopreservation of embryonic stem (ES) cells is essential to establish them as a resource for regenerative therapy. We evaluated survival adhesion rate, cell structure, gene expression, and multipotency of frozen and thawed embryoid bodies (EBs) derived from mouse ES cells. EBs were cryopreserved using the BICELL and the Program Freezer. After one week the EBs were thawed and cultured. EBs prepared in the Program Freezer had the highest survival adhesion (Program Freezer; 55-69%, BICELL; 30-38%). Though many cells in the thawed EBs were damaged, some were not, especially those prepared in the Program Freezer. RT-PCR analysis showed that genes characteristic of the three embryonic germ layers were expressed in thawed EBs cultured for one week. EBs transplanted into mice formed teratomas consisting of cells derived from the three germ layers. In conclusion, EBs frozen in the Program Freezer had higher survival adhesion rates compared to the BICELL and formed differentiated cells characteristic of the three embryonic germ layers.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cryopreservation
/
Embryonic Stem Cells
Limits:
Animals
Language:
En
Journal:
Cryobiology
Year:
2007
Document type:
Article
Affiliation country:
Japan