Regulation of geminin functions by cell cycle-dependent nuclear-cytoplasmic shuttling.
Mol Cell Biol
; 27(13): 4737-44, 2007 Jul.
Article
in En
| MEDLINE
| ID: mdl-17470552
ABSTRACT
The geminin protein functions both as a DNA rereplication inhibitor through association with Cdt1 and as a repressor of Hox gene transcription through the polycomb pathway. Here, we report that the functions of avian geminin are coordinated with and regulated by cell cycle-dependent nuclear-cytoplasmic shuttling. In S phase, geminin enters nuclei and inhibits both loading of the minichromosome maintenance (MCM) complex onto chromatin and Hox gene transcription. At the end of mitosis, geminin is exported from nuclei by the exportin protein Crm1 and is unavailable in the nucleus during the next G(1) phase, thus ensuring proper chromatin loading of the MCM complex and Hox gene transcription. This mechanism for regulating the functions of geminin adds to distinct mechanisms, such as protein degradation and ubiquitination, applied in other vertebrates.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cell Cycle
/
Cell Nucleus
/
Chickens
/
Cytoplasm
/
Avian Proteins
Limits:
Animals
Language:
En
Journal:
Mol Cell Biol
Year:
2007
Document type:
Article
Affiliation country:
Germany