An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp.: functional expression and enzymatic characterization.

Ooi, Toshihiko; Shibata, Takeshi; Sato, Reiko; Ohno, Hiroaki; Kinoshita, Shinichi; Thuoc, Tran Linh; Taguchi, Seiichi

Ooi, Toshihiko; Shibata, Takeshi; Sato, Reiko; Ohno, Hiroaki; Kinoshita, Shinichi; Thuoc, Tran Linh; Taguchi, Seiichi.

Affiliation

Ooi T; Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, 060-8628, Sapporo, Japan. ooi@dove-mc.eng.hokudai.ac.jp

Appl Microbiol Biotechnol ; 75(2): 377-86, 2007 May.

Article in En | MEDLINE | ID: mdl-17546472

Subject(s)

Bacillus/enzymology; NADH, NADPH Oxidoreductases/genetics; NADH, NADPH Oxidoreductases/metabolism; NAD/metabolism; Aerobiosis; Bacillus/classification; Bacillus/genetics; Bacillus/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Flavoproteins/metabolism; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; NADH, NADPH Oxidoreductases/chemistry; NADH, NADPH Oxidoreductases/isolation & purification; Nitroreductases; Sequence Analysis, DNA; Soil Microbiology; Substrate Specificity; Temperature

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Collection: 01-internacional Database: MEDLINE Main subject: Bacillus / NAD / NADH, NADPH Oxidoreductases Language: En Journal: Appl Microbiol Biotechnol Year: 2007 Document type: Article Affiliation country: Japan Country of publication: Germany

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