Chromosome segregation control by Escherichia coli ObgE GTPase.
Mol Microbiol
; 65(2): 569-81, 2007 Jul.
Article
in En
| MEDLINE
| ID: mdl-17578452
ABSTRACT
Escherichia coli cells depleted of the conserved GTPase, ObgE, show early chromosome-partitioning defects and accumulate replicated chromosomes in which the terminus regions are colocalized. Cells lacking ObgE continue to initiate replication, with a normal ratio of the origin to terminus. Localization of the SeqA DNA binding protein, normally seen as punctate foci, however, was disturbed. Depletion of ObgE also results in cell filamentation, with polyploid DNA content. Depletion of ObgE did not cause lethality, and cells recovered fully after expression of ObgE was restored. We propose a model in which ObgE is required to license chromosome segregation and subsequent cell cycle events.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromosomes, Bacterial
/
Chromosome Segregation
/
Monomeric GTP-Binding Proteins
/
Escherichia coli Proteins
/
Escherichia coli
Language:
En
Journal:
Mol Microbiol
Journal subject:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
Year:
2007
Document type:
Article
Affiliation country:
United States