Critical role of P-selectin-dependent leukocyte recruitment in endotoxin-induced intestinal barrier dysfunction in mice.
Inflamm Res
; 56(5): 189-94, 2007 May.
Article
in En
| MEDLINE
| ID: mdl-17588134
ABSTRACT
OBJECTIVE:
To define the importance of leukocyte recruitment in endotoxin-induced gut permeability. MATERIALS ANDMETHODS:
31 male C57BL/6 mice were challenged with lipopolysaccharide (LPS). Ileal permeability was measured in Ussing chambers and leukocyte-endothelium interactions studied with intravital fluorescence microscopy after 18 h.RESULTS:
LPS caused a clear-cut increase in leukocyte accumulation and intestinal permeability. Immunoneutralisation of P-selectin not only reduced leukocyte recruitment significantly (54 % reduction) but also abolished endotoxin-induced intestinal leakage. Intestinal levels of pro-inflammatory chemokines increased markedly in response to LPS but were not influenced by inhibition of P-selectin in vivo.CONCLUSION:
The present study shows not only that endotoxin-induced leukocyte recruitment is mediated by P-selectin but also that sepsis-associated intestinal leakage in the gut is largely regulated by leukocyte accumulation. Thus, our novel data demonstrate a critical link between P-selectin-dependent leukocyte recruitment and gut barrier failure in endotoxemia.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
P-Selectin
/
Endotoxemia
/
Ileum
/
Intestinal Absorption
/
Leukocytes
Limits:
Animals
Language:
En
Journal:
Inflamm Res
Journal subject:
ALERGIA E IMUNOLOGIA
/
PATOLOGIA
Year:
2007
Document type:
Article
Affiliation country:
Sweden