High-performance liquid chromatographic separation of rolipram, bupivacaine and omeprazole using a tartardiamide-based stationary phase influence of flow rate and temperature on the enantioseparation.
J Chromatogr A
; 1162(1): 97-102, 2007 Aug 24.
Article
in En
| MEDLINE
| ID: mdl-17645885
ABSTRACT
Chromatographic separation of the chiral drugs rolipram, bupivacaine and omeprazole on a tartardiamide-based stationary phase commercially named Kromasil CHI-TBB is shown in this work. The effect of temperature on the chromatographic separation of the chiral drugs using the Kromasil CHI-TBB stationary phase was determined quantitatively so as to contribute toward the design for the racemic mixtures of the named compound by using chiral columns. A decrease in the retention and selectivity factors was observed, when the column temperature increased. Van't Hoff plots provided the thermodynamic data. The variation of the thermodynamic parameters enthalpy and entropy are clearly negative meaning that the separation is enthalpy controlled.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tartrates
/
Omeprazole
/
Bupivacaine
/
Chromatography, High Pressure Liquid
/
Chromatography, Ion Exchange
/
Rolipram
Type of study:
Prognostic_studies
Language:
En
Journal:
J Chromatogr A
Year:
2007
Document type:
Article
Affiliation country:
Brazil