Detection of equine herpesviruses in aborted foetuses by consensus PCR.
Vet Microbiol
; 126(1-3): 20-9, 2008 Jan 01.
Article
in En
| MEDLINE
| ID: mdl-17686590
ABSTRACT
The major role of EHV-1 in equine abortion is widely reported in the literature but the contribution of EHV-2, EHV-3, EHV-4 or EHV-5 remains less well documented. The objective of this study is to evaluate the contribution of these five different EHVs to equine abortion in a variety of biological tissues using a consensus polymerase chain reaction (PCR). The test was validated for specificity and sensitivity in horses before screening specimens from 407 foetuses, stillbirths and premature foals collected over a 2.5-year interval. Positive results obtained with this assay were compared to other EHV type-specific PCR or by sequencing. EHV-1 was identified as the major cause of abortion in French mares (59/407 cases). However, there was evidence to suggest some variation in the potential of EHV-1 strains to induce abortion. Indeed, DNA samples from EHV-2 (in three cases) and EHV-5 (in one case) inferred a role of these viruses in abortion. The presence of viral DNA from EHV-3 or EHV-4 strains was not detected in the specimens studied. The data obtained suggest that the consensus herpesvirus PCR is an efficient screening tool. In association with a specific PCR, the test provides a rapid identification of the type of herpesvirus involved in abortion and is useful for routine diagnostic tests as it allows the identification of herpesviruses other than the EHV-1 strain.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymerase Chain Reaction
/
Varicellovirus
/
Herpesviridae Infections
/
Abortion, Veterinary
/
Aborted Fetus
/
Horse Diseases
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Vet Microbiol
Year:
2008
Document type:
Article
Affiliation country:
France