A synergistic effect of a combined bivalent DNA-protein anti-HIV-1 vaccine containing multiple T- and B-cell epitopes of HIV-1 proteins.
Mol Immunol
; 45(3): 661-9, 2008 Feb.
Article
in En
| MEDLINE
| ID: mdl-17869341
ABSTRACT
Immunogenic properties of the combined vaccine CombiHIVvac, comprising polyepitope HIV-1 immunogens, one being the artificial polyepitope protein TBI, containing the T- and B-cell epitopes from Env and Gag proteins, and the DNA vaccine construct pcDNA-TCI coding for the artificial protein TCI, carrying over 80 T-cell epitopes (both CD4+ CTL and CD8+ Th) from Env, Gag, Pol, and Nef proteins, are studied in this work. The data reported demonstrate clearly that a combination of two B- and T-cell immunogens (TBI and TCI) in one construct results in a synergistic increase in the antibody response to both TBI protein and the proteins from HIV-1 lysate. The level of antibodies induced by immunization with the constructs containing either immunogen alone (TBI protein or the plasmid pcDNA-TCI) was significantly lower as compared to that induced by the combined vaccine. The analysis performed suggests that the presence of CD4+ T-helper epitopes, which can be presented by MHC class II, in the protein TCI may be the main reason underlying the increased synthesis of antibodies to TBI protein due to a CD4-mediated stimulation of B-cell proliferation and differentiation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Viral Proteins
/
HIV-1
/
AIDS Vaccines
/
Epitopes, T-Lymphocyte
/
Epitopes, B-Lymphocyte
/
Antibodies, Viral
/
Antibody Formation
Limits:
Animals
Language:
En
Journal:
Mol Immunol
Year:
2008
Document type:
Article
Affiliation country:
RUSSIA