Virus-induced gene silencing for functional analysis of selected genes.
Plant Cell Rep
; 27(2): 209-19, 2008 Feb.
Article
in En
| MEDLINE
| ID: mdl-17938933
ABSTRACT
Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Viruses
/
Plants
/
Gene Silencing
Language:
En
Journal:
Plant Cell Rep
Journal subject:
BOTANICA
Year:
2008
Document type:
Article
Affiliation country:
Singapore