Robust, persistent transgene expression in human embryonic stem cells is achieved with AAVS1-targeted integration.

Smith, Joseph R; Maguire, Sean; Davis, Lesley A; Alexander, Morgan; Yang, Fentang; Chandran, Siddharthan; ffrench-Constant, Charles; Pedersen, Roger A

Smith, Joseph R; Maguire, Sean; Davis, Lesley A; Alexander, Morgan; Yang, Fentang; Chandran, Siddharthan; ffrench-Constant, Charles; Pedersen, Roger A.

Affiliation

Smith JR; Department of Surgery, Cambridge Institute for Medical Research, Wellcome Trust/MRC Building, Addenbrooke's Hospital, Hills Road, Cambridge CB2 OXY, United Kingdom. jrs68@cam.ac.uk

Stem Cells ; 26(2): 496-504, 2008 Feb.

Article in En | MEDLINE | ID: mdl-18024421

Subject(s)

Dependovirus/genetics; Embryonic Stem Cells/metabolism; Cell Differentiation; Cell Line; Chromosomes, Human, Pair 19/genetics; Embryonic Stem Cells/cytology; Gene Expression; Gene Silencing; Genes, Reporter; Green Fluorescent Proteins/genetics; Humans; In Situ Hybridization, Fluorescence; Phenotype; Recombinant Proteins/genetics; Transfection; Virus Integration/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Dependovirus / Embryonic Stem Cells Limits: Humans Language: En Journal: Stem Cells Year: 2008 Document type: Article Affiliation country: United kingdom Country of publication: United kingdom

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