Effect of collision-activated dissociation gas and collision energy on the fragmentation of dipyridamole and its rapid and sensitive liquid chromatography/electrospray ionization tandem mass spectrometric determination in human plasma.
Rapid Commun Mass Spectrom
; 22(4): 511-8, 2008.
Article
in En
| MEDLINE
| ID: mdl-18205248
ABSTRACT
The effect of nitrogen as the collision-activated dissociation (CAD) gas on the fragmentation of dipyridamole was investigated in the range of 10-90 eV collision energy. The results support the collision model reported elsewhere, that the degree of ion fragmentation increases with the increasing mass of the collision gas. A simple, sensitive and high-throughput liquid chromatography/tandem mass spectrometry (LC/MS/MS) method has been developed for the determination of dipyridamole, a platelet aggregation inhibitor in human plasma, using granisetron as internal standard (IS). The method involved liquid-liquid extraction of the analyte and IS from 0.5 mL human plasma with diethyl ether. The chromatographic separation was achieved under isocratic conditions and the ion transitions for dipyridamole (m/z 505.40 --> 429.60) and the IS (m/z 313.10 --> 138.20) were monitored on a triple quadrupole mass spectrometer, operating in positive ion multiple reaction monitoring (MRM) mode. The method was validated over the concentration range 5.1-4499.1 ng/mL for dipyridamole. The method was rugged and rapid with a total run time of 1.2 min. It was successfully applied to a pivotal bioequivalence study in 67 healthy human subjects after oral administration of a 75 mg extended release formulation under fasting condition.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Chemical Analysis
/
Chromatography, High Pressure Liquid
/
Artifacts
/
Spectrometry, Mass, Electrospray Ionization
/
Dipyridamole
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Rapid Commun Mass Spectrom
Year:
2008
Document type:
Article
Affiliation country:
India