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Purification of fibronectin.
Akiyama, S K.
Affiliation
  • Akiyama SK; Chapel Hill, North Carolina, USA.
Curr Protoc Cell Biol ; Chapter 10: Unit 10.5, 2001 May.
Article in En | MEDLINE | ID: mdl-18228299
This unit describes the purification of the multifunctional adhesive glycoprotein fibronectin from plasma or of cell-derived fibronectin from cell surfaces and from conditioned medium. Fibronectin can be used in cell adhesion and migration assays, and can be obtained in relatively high purity using simple affinity chromatography techniques.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fibronectins / Culture Media, Conditioned / Extracellular Matrix Limits: Humans Language: En Journal: Curr Protoc Cell Biol Year: 2001 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fibronectins / Culture Media, Conditioned / Extracellular Matrix Limits: Humans Language: En Journal: Curr Protoc Cell Biol Year: 2001 Document type: Article Affiliation country: United States Country of publication: United States