Enterococcus faecalis from newborn babies regulate endogenous PPARgamma activity and IL-10 levels in colonic epithelial cells.
Proc Natl Acad Sci U S A
; 105(6): 1943-8, 2008 Feb 12.
Article
in En
| MEDLINE
| ID: mdl-18234854
ABSTRACT
The postembryonic development of the gastrointestinal tract is subject to regulation by the colonizing microbiota. This maturation process requires the commensal bacteria to cross-talk with host cells by way of recognizing receptors and inducing signaling pathways to activate transcription factors such as the nuclear receptors. Here, we show that in colonic cell lines and in primary colonic cells, Enterococcus faecalis isolated from newborn babies possess the ability to regulate peroxisome proliferator-activated receptor-gamma1 (PPARgamma1) activity through phosphorylation. This results in elevated DNA binding and transcriptional activation of downstream target genes, including IL-10, a cytokine known to modulate innate immune function. Furthermore, phosphorylation appears tightly regulated as phospho-PPARgamma1 becomes an immediate substrate for degradation possibly to curtail any extended transactivation. The involvement of PPARgamma1 in a myriad of physiological processes further confirms that microflora-driven regulation might be important for a number of homeostatic strategies in the gut.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Interleukin-10
/
Enterococcus faecalis
/
Colon
/
PPAR gamma
Limits:
Humans
/
Newborn
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2008
Document type:
Article
Affiliation country:
Sweden