Development of novel magnetic nano-carriers for high-performance affinity purification.
Colloids Surf B Biointerfaces
; 64(2): 162-9, 2008 Jul 15.
Article
in En
| MEDLINE
| ID: mdl-18313904
ABSTRACT
We developed novel magnetic nano-carriers around 180 nm in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nano-carriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Chromatography, Affinity
/
Drug Delivery Systems
/
Nanoparticles
/
Magnetics
Language:
En
Journal:
Colloids Surf B Biointerfaces
Journal subject:
QUIMICA
Year:
2008
Document type:
Article
Affiliation country:
Japan